SIMPLE METHODS FOR "STAINING WITH LEAD" AT HIGH pH IN ELECTRON MICROSCOPY
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SIMPLE METHODS FOR "STAINING WITH LEAD" AT HIGH pH IN ELECTRON MICROSCOPY
The lead hydroxide stain of Watson (1958) used for increasing contrast in thin sections for electron microscopy has found acceptance in many laboratories. However, this stain has an unfortunate tendency to form precipitates (probably of lead carbonate (5)) on exposure to the air, thus contaminating the sections and irritating the observer. This drawback has led to the development of several mod...
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Aqueous solutions of lead salts (1, 2) and saturated solutions of lead hydroxide (1) have been used as stains to enhance the electron-scattering properties of components of biological materials examined in the electron microscope. Saturated solutions of lead hydroxide (1), while staining more intensely than either lead acetate or monobasic lead acetate (l , 2), form insoluble lead carbonate upo...
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Epon epoxy resin was introduced as an embedding medium for biological electron microscopy largely through the independent efforts of Finck (1) and Luft (2). The usual recipe involves three components: Epon 812 resin (Epikote 812, in England), an anhydride hardener, and an accelerator such as DMP-30. Since the combination of Epon with dodecenyl succinic anhydride (DDSA) was thought to produce a ...
متن کاملA Simplified Method of Staining Thin Sections of Biological Material with Lead Hydroxide for Electron Microscopy
A method of staining thin sections with lead hydroxide which enhances both resolution and contrast was described by M. L. Watson (1958) and is now undoubtedly in routine use in many laboratories. It is the purpose of this report to present a simplification of this method which eliminates the lengthy procedure of preparing the lead hydroxide reagent and the necessity of maintaining a COrfree atm...
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A n a q u e o u s so lu t ion of u r a n y l ace t a t e was first e m p l o y e d as a n e l ec t ron s ta in by W a t s o n (1958). W e h a v e f o u n d t h a t this s ta in , a l t h o u g h f r e q u e n t l y effective, is capr ic ious . T h e s ta in m a y no t inc rease con t r a s t of a g iven spec imen , a n d m a y , in o the r ins tances , l eave u n d e s i r a b l e c o n t a m i...
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ژورنال
عنوان ژورنال: The Journal of Biophysical and Biochemical Cytology
سال: 1961
ISSN: 1540-8140,0021-9525
DOI: 10.1083/jcb.11.3.729